Protein Complex Identification and quantitative complexome by CN-PAGE

Charting the protein complexome in yeast by mass spectrometry.

It has become evident over the past few years that many complex cellular processes, including control of the cell cycle and ubiquitin-dependent proteolysis, are carried out by sophisticated multisubunit protein machines that are dynamic in abundance, post-translational modification state, and composition. To understand better the nature of the macromolecular assemblages that carry out the cell ...

Isolation and identification of excretory-secretory and somatic antigens from the Oestrus ovis larvae by SDS-PAGE and immunoblotting

Oestrus ovis is an economically important parasite of small ruminants and a zoonotic parasite with many reports of ophthalmomyiasis in human from Iran and other countries. The aim of the peresent studywas the isolation and identification of excretory-secretory (ES) and somatic (S) antigens of O. ovis second and third stage larvae (L2, L3) collected from Arabi sheep breeds loca...

Immune complexome analysis.

Immune complexes (ICs) are produced during an immune response and may reflect some aspects of an ongoing immune response. Therefore, the identity of antigens incorporated into ICs provides the information that in the future may aid in the development of diagnosis and treatment strategies for autoimmune diseases, infection, cancer, and transplantation therapy, and this information might be more ...

Identification and quantitative determination of flavonoids in the aerial parts of Teucrium polium by HPLC

Review of literature shows that large varieties of compounds have been found to bear hypoglycemic action. For instance, glycosides afford active principles that lower blood sugar in test animals. Following the I.V. administration of a 4 percent decoction of the dried T. polium herb, a definite reduction of blood sugar level has been exhibited. With reference to PDR for Herbal Medicines for the ...

Identification of electrophoretic proteins pattern of Fusaium verticillioides isolates from maize by SDS-PAGE.